Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues
Tissue Engineering and Regenerative Medicine
;
(6): 29-38, 2019.
Article
in English
| WPRIM
| ID: wpr-742387
ABSTRACT
BACKGROUND:
Latest tissue engineering strategies for musculoskeletal tissues regeneration focus on creating a biomimetic microenvironment closely resembling the natural topology of extracellular matrix. This paper presents a novel musculoskeletal tissue scaffold fabricated by hybrid additive manufacturing method.METHODS:
The skeleton of the scaffold was 3D printed by fused deposition modeling, and a layer of random or aligned polycaprolactone nanofibers were embedded between two frames. A parametric study was performed to investigate the effects of process parameters on nanofiber morphology. A compression test was performed to study the mechanical properties of the scaffold. Human fibroblast cells were cultured in the scaffold for 7 days to evaluate the effect of scaffold microstructure on cell growth.RESULTS:
The tip-to-collector distance showed a positive correlation with the fiber alignment, and the electrospinning time showed a negative correlation with the fiber density. With reinforced nanofibers, the hybrid scaffold demonstrated superior compression strength compared to conventional 3D-printed scaffold. The hybrid scaffold with aligned nanofibers led to higher cell attachment and proliferation rates, and a directional cell organization. In addition, there was a nonlinear relationship between the fiber diameter/density and the cell actinfilament density.CONCLUSION:
This hybrid biofabrication process can be established as a highly efficient and scalable platform to fabricate biomimetic scaffolds with patterned fibrous microstructure, and will facilitate future development of clinical solutions for musculoskeletal tissue regeneration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Skeleton
/
Tissue Engineering
/
Biomimetics
/
Extracellular Matrix
/
Tissue Scaffolds
/
Microtechnology
/
Nanofibers
/
Fibroblasts
/
Printing, Three-Dimensional
Limits:
Humans
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2019
Type:
Article
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