Application value of plasma MMP-9, HBP and IL-27 combined detection in the early diagnosis of sepsis / 国际检验医学杂志

ABSTRACT

Objective To investigate the value of combined detection of plasma matrix metalloproteinase 9 (MMP-9), heparin binding protein (HBP) and interleukin 27 (IL-27) in the early diagnosis of sepsis.Methods A total of 55patients with sepsis in the ICU of People′s Hospital of Chongqing Banan District were selected and divided into sepsis group (24cases), severe sepsis group (17cases) and septic shock group (14patients) according to the severity of illness.Meanwhile, 21patients with non-sepsis local infection were selected as the control group.Record the plasma levels of MMP-9, HBP, IL-27relevant test data, acute physiology and chronic health status scoring system-Ⅱ (APACHE-Ⅱ) scores on admission.Pearson correlation test was used to analyze the correlation between plasma MMP-9, HBP and IL-27levels with PCT and APACHE-Ⅱ.Multivariate logistic regression analysis was used to analyze the correlation between MMP-9, HBP, IL-27and sepsis.The receiver operating characteristic curve (ROC) was used to analyze the combined detection of MMP-9, HBP, and IL-27for the early diagnosis of sepsis.Results The plasma levels of MMP-9, HBP and IL-27in patients with sepsis were significantly higher than those in the control group (P＜0.05).The levels of MMP-9, HBP and IL-27 were positively correlated with serum PCT (rMMP-9=0.534, rHBP=0.693, rIL-27=0.627, P＜0.05) and APACHE-Ⅱ (rMMP-9=0.574, rHBP=0.723, rIL-27=0.669, P＜0.05).Multivariate logistic regression analysis showed that MMP-9, HBP and IL-27were independent risk factors for sepsis (P＜0.05).ROC curve analysis suggested that the area under the curve (AUC) of MMP-9, HBP, and IL-27were 0.764 (95％CI=0.755-0.916), 0.713 (95％CI=0.627-0.795) and 0.871 (95％CI=0.762-0.936), combined detection of MMP-9, HBP and IL-27had an AUC value of 0.891 (95％CI=0.822-0.943), which were significantly higher than single detection of MMP-9, HBP and IL-27 (P＜0.05).Conclusion Combined detection of plasma MMP-9, HBP and IL-27has a good application value for early diagnosis of sepsis.