Effect of Danhong injection on serumα-klotho protein, lipid metabolism and atherosclerotic plaque in patients with chronic renal failure undergoing hemodialysis / 国际检验医学杂志

ABSTRACT

Objective To investigate the effect of Danhong injection on serumα-klotho protein, lipid metabolism and atherosclerotic plaque in patients with chronic renal failure undergoing hemodialysis.Methods A total of 100 patients with chronic renal failure treated in the hospital from February 2013 to April 2016 were selected and randomly divided into the control group and the observation group with 50 cases in each group.The two groups were treated with hemodialysis, and the observation group was additionally treated with Danhong injection.Changes in serumα-klotho protein, fibroblast growth factor 23 (FGF-23), lipid metabolism indexes, such as total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C), renal function indexes, such as serum creatinine (Scr), blood urea nitrogen (BUN) and endogenous creatinine clearance rate (Ccr) in the two groups were determined before and after treatment.Carotid artery ultrasonography was performed to observe changes in the size, number and thickness of atherosclerotic plaques and carotid intima-media thickness (IMT) before and after treatment.Results FGF-23, TC, TG, Scr and BUN in the observation group were decreased after 8 weeks of treatment[ (1 526.25±46.87) pg/mL, (5.21±1.23) mmol/L, (1.26±0.23) mmol/L, (390.11±52.26) μmol/L, (14.11±3.26) mmol/L vs. (1 768.74±54.26) pg/mL, (5.93±0.26) mmol/L, (1.63±0.14) mmol/L, (443.26±47.85) μmol/L, (18.52±2.79) mmol/L], whileα-Klotho protein, HDL-C and Ccr were increased[ (790.44±40.61) pg/mL, (1.21±0.21) mmol/L, (37.41±3.26) mL/min vs. (662.25±76.54) pg/mL, (1.00±0.29) mmol/L, (33.51±3.41) mL/min].Compared with those in the control group, there were statistically significant differences (t=23.914, 4.049, 9.716, 5.304, 7.267, 10.461, 4.147, 5.845, P＜0.05).In 6 months of follow-up, plaques shrank, the thickness of plaque and IMT decreased, and the number of plaques decreased in the observation group[ (0.06±0.01) cm2, (1.11±0.23) mm, (1.01±0.23) mm, (2.86±0.51) vs. (0.10±0.06) cm2, (1.87±0.49) mm, (1.43±0.20) mm, (3.41±1.03) ].Compared with those in the control group, the differences were statistically significant (t=4.649, 9.928, 9.743, 3.383, all P＜0.05).Conclusion Danhong injection can increase the expression level ofα-klotho protein in serum of patients with chronic renal failure undergoing hemodialysis, down regulate the expression of FGF-23, improve lipid metabolism and renal function in patients, reduce the degree of atherosclerosis, the number of patches and the thickness of plaque.