Comparison of Hematopoietic Stem Cell Activity in Normal Bone Marrow, in vivo G-CSF Stimulated Bone Marrow, and G-CSF-Mobilized Peripheral Blood / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 157-167, 2000.
Article
in Korean
| WPRIM
| ID: wpr-74357
ABSTRACT
BACKGROUND:
Classically, bone marrow (BM) has been the sole source of hematopoietic stem cell transplantation, but limitations of conventional bone marrow transplantation have stimulated a search for alternative sources of stem cells.METHODS:
We compared hematopoietic stem cell activity of normal bone marrow (BM), in vivo G-CSF-stimulated bone marrow (G-CSF BM), and G-CSF-mobilized peripheral blood (G-CSF PB) by immunophenotyping, clonogeneicity, and long-term culture-initiating cell (LTC-IC) analysis. RESLUTS The average numbers of CD34+/HLA-DR- cells after CD34+ cells isolation from each stem cell source were 59.64 +/- 8.70%, 91.39 +/- 1.98%, and 95.75 +/- 2.08% in normal BM, G-CSF BM, and G-CSF PB, respectively (normal BM vs. G-CSF BM, normal BM vs. G-CSF PB, p<0.0001). And the average numbers of CD34+/CD38- cells were 66.23 +/- 9.33%, 95.08+/- 2.09%, and 91.76 +/- 4.59% in normal BM, G-CSF BM, and G-CSF PB, respectively (normal BM vs. G-CSF BM, normal BM vs. G-CSF PB, p<0.0001). The numbers of CFU-GM was significantly higher in G-CSF PB (53.2 +/- 4.05) and G-CSF BM (52.5 +/- 3.63) than that of normal BM (31.3+/- 5.50) (p<0.0001). Also the numbers of CFU-GEMM and CFU-Mk were also significantly higher in G-CSF PB (110.3 +/- 8.79 and 13.3 +/- 1.49) and G-CSF BM (109.7 +/- 10.78 and 11.2 +/- 1.69) than that of normal BM (48.8 +/- 1.48 and 8.5 +/- 1.72) (p<0.05). Comparison of LTC-IC in the three sources of stem cells showed that G-CSF PB and G-CSF BM were superior to normal BM at five weeks of culture (p<0.05).CONCLUSIONS:
These data suggest that the amount of both early progenitor cells and late progenitor cells in G-CSF PB and G-CSF BM are higher than that of normal BM. And our results further support that the higher stem cell transplantation using G-CSF-mobilized PB and in vivo G-CSF-stimulated BM can lead to more rapid and sustained engraftment even in cases of high risk of rejection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Bone Marrow
/
Hematopoietic Stem Cells
/
Granulocyte Colony-Stimulating Factor
/
Immunophenotyping
/
Bone Marrow Transplantation
/
Hematopoietic Stem Cell Transplantation
/
Myeloid Progenitor Cells
/
Stem Cell Transplantation
/
Granulocyte-Macrophage Progenitor Cells
Language:
Korean
Journal:
Korean Journal of Blood Transfusion
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS