Effects of curcumin on spinal astrocyte damage induced by H2O2 through Notch signaling pathway / 中国免疫学杂志

ABSTRACT

Objective: To investigate the effect of curcumin on Asrocytes and Notch signaling pathway in spinal cord injury. Methods Spinal cord Asrocytes was isolated and cultured, and cells were treated with 0. 1, 0. 2, 0. 4, 0. 6, 0. 8, 1 mmol/L hydrogen peroxide ( H2O2), the cell viability was measured by CCK8, AS damage model was established.Methods: The experimental grouping was control group, H2O2 group and H2O2+curcumin group, cell apoptosis and ROS content were detected by flow cytometry; the content of IL-6 and TNF-α were detected by ELISA kit; the expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein were detected by Western blot. Results: The activity of AS was inhibited by different concentrations of H2O2, and the inhibition rate of cell increased with the increase of H2O2 concentration. IC50 for 0. 4 mmol/L's H2O2 was selected to establish a damage model; compared with the normal control group, the apoptosis rate, the content of ROS, IL-6, TNF-α and expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein expression were increased significantly in H2O2 group, compared with H2O2 group, the apoptosis rate, the content of ROS, IL-6, TNF-α and expression of Cleaved Caspase3, Bax, Notch1 and Hes1 protein expression were lower significantly in H2O2+curcumin group ( P＜0. 05). Conclusion: Curcumin can reduce the apoptosis of Asrocytes, the content of ROS and the contents of inflammatory factors IL-6 and TNF-α in spinal cord by down regulating the Notch signaling pathway, thereby protecting spinal cord injury.