Effect of Noscapine on the Biological Function of A549 Cells / 中国医科大学学报

ABSTRACT

Objective To explore the effect of noscapine on the biological function of A549 cells and its underlying mechanism. Methods Concentrations of 10 μmol/L and 20 μmol/L of noscapine were used on the A549 cells. The effect of noscapine on their proliferation was observed via the MTT assay. Western blotting and real time PCR were used to detect the effects on Cyclin D1, BCL-2, and MMP2. The Hoechst 33258 staining assay was used to observe the effects of noscapine on the apoptosis of A549 cells. The metastasis of A549 cells was detected by using the Transwell assay. Results The MTT assay showed that the rate of inhibition of cell proliferation after treatment with 10 μmol/L and 20 μmol/L noscapine were (32.98±1.09) ％ and (49.56±3.98) ％, with a significant difference between the values (P ＜ 0.05). Hoechst 33258 staining evinced that noscapine promoted apoptosis, while the Transwell assay displayed that noscapine inhibited A549 cell metastasis. Western blotting and real time PCR indicated that the expression of Cyclin D1, BCL-2, and MMP2 in A549 cells was inhibited by noscapine. Conclusion Noscapine can inhibit the growth and metastasis of the lung cancer cell line A549 and promote the apoptosis of A549 cells. In conclusion, noscapine can potentially be used as a chemotherapeutic drug for lung cancer.