Characteristics of mRNA level of interleukin 12 and intercellular adhesion molecule 1 and monocyte chemotactic protein 3 in human nasal epithelial cells / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 1068-1070, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-746531
ABSTRACT
OBJECTIVE@#To investigate the mRNA level of IL-12, ICAM-1 and MCP-3 in human nasal epithelial cells.@*METHOD@#Firstly, human primary nasal epithelial cells were cultured, and then 4 pairs of primers were designed for detecting mRNA level of IL-12, ICAM-1 and MCP-3. The 938 bp PCR products of GAPDH were used as internal standards. The mRNA expression levels of IL-12, ICAM-1 and MCP-3 in primary nasal epithelial cells was measured with semi-quantitative reverse transcription-polymerase chain reaction.@*RESULT@#The round or irregular primary nasal epithelial cells were observed sticking to the bottom of cell culture plates under 400 times optical microscope. The expressions of IL-12 p35, ICAM-1 and MCP-3 mRNA were found in primary nasal epithelial cells while IL-12 p40 subunit was not detected.@*CONCLUSION@#IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells, whereas effective IL-12 with integrity is not present in nasal epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Cells, Cultured
/
Intercellular Adhesion Molecule-1
/
Cell Biology
/
Epithelial Cells
/
Interleukin-12 Subunit p35
/
Chemokine CCL7
/
Genetics
/
Metabolism
/
Nasal Mucosa
Limits:
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2008
Type:
Article
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