Effect of apoptosis of vesicular stomatitis virus on tumor cells of nasopharyngeal carcinoma in vivo / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 995-997, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-746559
ABSTRACT
OBJECTIVE@#To investigate apoptosis induced by vesicular stomatitis virus (VSV) in HNE-1 cancer cells of human nasopharyngeal carcinoma by models of nude mice-BALB/c in vivo.@*METHOD@#HNE-1 cells are collected from culture bottle and infected infra right-side post-back epithelium of nude mice-BALB/c (6 x 10(6) cells/0.1 ml/each mice) to create HNE-1 tumor models of nude mice-BALB/c. When the diameter of HNE-1 tumors is 5 to 8 millimeter, HNE-1 tumor models are treated with VSV (1 x 10(8) pfu /ml) or Saline. By Hoechst 33258-staining under fluorescence microscope, induction of apoptosis by VSV in HNE-1 tumor models are recorded and studied, compared with that by Saline in HNE-1 tumor models in vivo.@*RESULT@#Compared with control group of saline, apoptosis of HNE-1 cancer cells of human nasopharyngeal carcinoma increase apparently in the remaining tumor cells of nude mice treated by VSV (P < 0.01).@*CONCLUSION@#The present study suggests that the treatment with VSV could augment the apoptosis cells of human nasopharyngeal carcinoma in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Nasopharyngeal Neoplasms
/
Apoptosis
/
Vesiculovirus
/
Xenograft Model Antitumor Assays
/
Cell Line, Tumor
/
Oncolytic Virotherapy
/
Mice, Inbred BALB C
/
Mice, Nude
/
Neoplasm Transplantation
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2008
Type:
Article
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