The study on growth and proliferation of neural stem cells from rats in vitro / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 747-750, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-746592
ABSTRACT
OBJECTIVE@#To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.@*METHOD@#NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.@*RESULT@#NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.@*CONCLUSION@#NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Differentiation
/
Cells, Cultured
/
Embryology
/
Culture Media, Serum-Free
/
Rats, Sprague-Dawley
/
Cell Biology
/
Multipotent Stem Cells
/
Cell Proliferation
/
Embryo, Mammalian
/
Neural Stem Cells
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2008
Type:
Article
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