The Efficacy and Safety of the Domestic Organ Preservation Solutions, SNU-I and SNU-II, Using the Pig Renal Autotransplantation Model / 대한이식학회지

ABSTRACT

Organ preservation solutions currently in use have been quite successful clinically, but it is true that improvements can still be made in preservation time and postpreservation function of the graft. The aim of this study is to compare the effectiveness of two domestic organ preservation solutions, SNU-I and SNU-II, with that of UW solution in a swine autotransplantation model. SNU-I is a domestic reproduction of UW solution and SNU-II is an theoretically improved regimen with the addition of pyruvate and aspartate. Unilateral nephrectomy was done in 14 female pigs and the grafts were cold-preserved with UW (n=3), SNU-I (n=6) and SNU-II (n=5) solution for 24 hours before being autotransplanted back to the same animal. The groups were compared in posttransplant serum BUN/creatinine level and in the degree of tissue injury in graft specimens taken 14 days after the transplantation. The peak values for the BUN/creatinine level were the highest in the SNU-II group, reaching 80 mg/dl for BUN and 6.43 mg/dl for creatinine. Those for the UW and SNU-I groups were lower, but the differences were not statistically significant. The tissues were embedded in paraffin, stained with H/E and then were subjected to light microscopic examination (X200). When the degree of tissue injury was scored on the items of tubular necrosis, intratubular cell detachment, and brush border integrity, the SNU-II group displayed higher scores than the other two. The SNU-II group was also given higher scores in the degree and aggressiveness of the inflammatory process, but the difference between the three groups were not statistically significant in all items. In conclusion, UW and SNU-I solutions appear to have the same potency as organ preservation solutions. Further investigations are needed for the efficacy of SNU-II solution, including longer term preservation or warm ischemia models.