The effect of oxidative stress on aorta endothelial cell in chronic intermittent hypoxia animal model / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To make a chronic intermittent hypoxia (CIH) animal model and explore the relationships of oxidative stress and the injury of aorta endothelial cell on the CIH rat model.@*METHOD@#Twenty-four sex maturation rat were randomly Divided into unhandled control group(normal feeding for 4 weeks, UC group), CIH group (CIH for 4 weeks), reoxygenation group (normal feeding for 4 weeks after CIH for 4 weeks, RH group). Determined the activity of superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) in blood serum of all groups. Dislodged rat's aorta, observed the appearance of aorta endothelial cell and the expression of endothelial nitric oxide synthase (eNOS).@*RESULT@#Compared with UC group, in CIH group the activities of SOD and NO were significantly lower, but MDA significantly higher (all of P 0.05).@*CONCLUSION@#Oxidative stress played a role in the injury of aorta endothelial cell on ob chronic intermittent hypoxia rat modle.