Expression and clinical significance of MMP9 in sinonasal squamous cell carcinoma / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#We detected expression of MMP9 to discuss its role in the occurrence and development of sinonasal squamous cell carcinoma.@*METHOD@#The immunohistochemical staining, real-time PCR and Western blot were used to measure the expression of MMP9 in sinonasal squamous cell carcinoma tissues (Experimental group) and corresponding normal mucosa tissues (Control group). Relationship between MMP9 and the main clinical features of patients with sinonasal squamous cell carcinoma was analysed.@*RESULT@#Positive expression rates of MMP9 in sinonasal squamous cell carcinoma tissues and corresponding normal mucosa tissues were 81. 25% and 18. 52% respectively. Positive expression rate of MMP9 was not significantly correlated with patient's age and gender (P>0. 05), but correlated with pathological type (P<0. 05). The expression of MMP9 mRNA in sinonasal squamous carcinoma tissues was 30. 66 times of tissues adjacent to carcinoma (P<0. 05). Western blot analysis also showed that the expression of MMP9 protein in squamous carcinoma tissues was significantly higher than tissues adjacent to carcinoma (P<. 05).@*CONCLUSION@#The expression of MMP9 was significantly higher in the sinonasal squamous cell carcinoma and correlated with the degree of differentiation. The results suggest that MMP9 may play a role in the occurrence and development of sinonasal squamous cell carcinoma and degree of malignancy from the protein and cellular and molecular level. The higher degree of malignancy, the stronger expression.