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Effect of microRNA-205 on proliferation of laryngeal carcinoma cell line Hep-2 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 831-834, 2015.
Article in Chinese | WPRIM | ID: wpr-747287
ABSTRACT
OBJECTIVE@#To study the effect of microRNA-205 (miRNA-205) on proliferation of laryngeal carcinoma cell line Hep-2.@*METHOD@#The expressions of miRNA-205 in 27 cases laryngeal carcinoma tissues and adjacent normal tissues were detected by Real-time quantitative PCR, the expression of PTEN protein was detected by Western blot. The expressions of PTEN were detected by Western blot after miRNA-205 inhibitor or miRNA-205 mimics was transfected into Hep-2 cells and Hep-2 cells proliferation was measured by CCK-8 kit.@*RESULT@#The expression level of miRNA-205 was significantly higher in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01), and the expression of PTEN protein was lower in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01). The proliferation rate of Hep-2 cells was decreased significantly and the expression of PTEN protein in Hep-2 cells was increased significantly after miRNA-205 inhibitor was transfected into (P < 0.01), and the proliferation rate of Hep-2 cells was increased significantly and the expression of PTEN protein in Hep-2 cells was decreased significantly after miRNA-205 mimics was transfected into (P < 0.01).@*CONCLUSION@#miRNA-205 might promote the proliferation of Hep-2 cells by regulating the expression of PTEN.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Transfection / Laryngeal Neoplasms / MicroRNAs / Cell Line, Tumor / Cell Proliferation / PTEN Phosphohydrolase / Metabolism Limits: Humans Language: Chinese Journal: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Transfection / Laryngeal Neoplasms / MicroRNAs / Cell Line, Tumor / Cell Proliferation / PTEN Phosphohydrolase / Metabolism Limits: Humans Language: Chinese Journal: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Year: 2015 Type: Article