Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 633-637, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-747746
ABSTRACT
OBJECTIVE@#To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.@*METHOD@#Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.@*RESULT@#Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05).@*CONCLUSION@#Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Carcinoma, Squamous Cell
/
Transfection
/
Cell Movement
/
Blotting, Western
/
Guanine Nucleotide Exchange Factors
/
Cell Line, Tumor
/
Cell Proliferation
/
T-Lymphoma Invasion and Metastasis-inducing Protein 1
/
Squamous Cell Carcinoma of Head and Neck
Limits:
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2015
Type:
Article
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