Studies on the Effects of Monoclonal Antibodies to Mammalian Heat Shock Protein 60 (HSP 60) on Mouse Embryo Development In Vitro / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2216-2220, 2003.
Article
in Korean
| WPRIM
| ID: wpr-7479
ABSTRACT
OBJECTIVE:
To investigate the effects of antibodies to HSP 60 on the early mouse embryo development in vitro.METHODS:
The 175 late 2-cell mouse embryos were obtained from 6-7 week old female ICR mice. 5-10 embryos were placed in each well. The embryos were incubated in the Ham's F-10 medium supplemented with 100 microgram/mL of monoclonal antibody to HSP 60 (66), monoclonal mouse IgG1 (55), and medium alone (54), respectively, at 37degrees C in a 5% CO2 humidified air chamber, and mouse embryo developments were observed daily.RESULTS:
On day4, growth arrests were more prominent in anti-HSP 60 containing group compared to IgG1 containing group, medium only group (0% vs 16%, 14%), and these results were statistically significant (p=0.0032). Especially those inhibitory effects were observed in early stage of embryo development (day1) and these results were also statistically significant (31% vs 83%, 77%, p<0.0001). Moreover, we found out that cellular degenerations were more common in anti-HSP 60 containing group and this features were prominent on day2.CONCLUSION:
Anti-HSP 60 elicited a strong growth inhibitory and degenerative effect on early mouse embryo development. These findings suggest that HSP 60 may exert a protective effect against mouse embryo degeneration or apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin G
/
Apoptosis
/
Chaperonin 60
/
Embryonic Development
/
Embryonic Structures
/
Hot Temperature
/
Heat-Shock Proteins
/
Mice, Inbred ICR
/
Antibodies
/
Antibodies, Monoclonal
Limits:
Animals
/
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2003
Type:
Article
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