Construction and expression of the targeting super-antigen EGF-SEA fusion gene / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 638-656, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-748160
ABSTRACT
OBJECTIVE@#To construct expression vector for the SEA-EGF fusion gene.@*METHOD@#Clone the SEA gene and the EGF gene segment with PCR and RT-PCR independently, and connect this two genes by the bridge PCR. Insert the fusion gene EGF-SEA into the expression vector PET-44. Induced the secretion of the fusion protein SEA-EGF by the antileptic.@*RESULT@#The gene fragment encoding EGF and SEA mature peptide was successfully cloned. The fusion gene EGF-SEA was successfully constructed and was inserted into expression vector.@*CONCLUSION@#The new recombinant expression vector for fusion gene EGF-SEA is specific for head and neck cancer, laid the foundation for the further study of fusion protein SEA-EGF targeting immune therapy in head and neck tumors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Drug Therapy
/
Enterotoxins
/
Epidermal Growth Factor
/
Molecular Targeted Therapy
/
Genetic Vectors
/
Genetics
/
Head and Neck Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2014
Type:
Article
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