The effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 731-734, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-748208
ABSTRACT
OBJECTIVE@#To study the effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig.@*METHOD@#Thirty guinea pigs were randomly divided into control group (10), cisplatin group (10) and forsythiaside group (10). The ototoxicity model was done with intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days. Forsythiaside (25 mg/kg per day) was injected 30 min before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days. The saline instead of cisplatin was injected in normal control group. The distortion product otoacoustic emission (DPOAE) was detected before animals were killed. The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction (RT-PCR).@*RESULT@#DPOAE amplitudes in cisplatin group was significantly lower than in control group (P < 0.01). Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly (P < 0.05). The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group (P < 0.01). Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group.@*CONCLUSION@#Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cisplatin
/
Proto-Oncogene Proteins c-jun
/
Cochlea
/
Toxicity
/
Glycosides
/
Guinea Pigs
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2014
Type:
Article
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