Microsatellite alternation in laryngeal squamous cell carcinomas / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 241-244, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-748253
ABSTRACT
OBJECTIVE@#To examine the microsatellite instability and loss of heterozygosity in the pathogenic mechanism of laryngeal squamous cell carcinomas.@*METHOD@#Forty cases squamous cell carcinomas of larynx were analyzed by comparing tumorous tissues and normal tissues around with 3 microsatellite markers from chromosome 3, 5 and 11, using PCR and PGE-AgNO3 staining.@*RESULT@#Among the 40 cases of laryngeal squamous cell carcinomas, 87.5% (35/40) of samples showed microsatellite instability or loss of heterozygosity in one to three microsatellite markers. High frequent microsatellite abnormal occurred at D5S592, it was 70% (28/40). Then the mutation rate of D3s1228 was 52.5% (21/40).@*CONCLUSION@#Our study revealed that tumor suppressor genes nearby chromosome 3p14 and 5q23 regions related to the pathogenesis of squamous cell carcinomas of larynx. A correlation between microsatellite alternation and stage of the tumor were found in D3s1228 and D5s592 chromosome regions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Carcinoma, Squamous Cell
/
Laryngeal Neoplasms
/
Genes, Tumor Suppressor
/
Loss of Heterozygosity
/
Microsatellite Instability
/
Genetics
/
Neoplasm Staging
Limits:
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2009
Type:
Article
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