Research of 10-23 DNAZyme inhibit the expression of eIF4E genes / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 552-554, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-748373
ABSTRACT
OBJECTIVE@#To explore the possibility of 10-23DNAzyme becoming a new gene therapy for laryngeal carcinoma treatment at the cell level.@*METHOD@#Thiosthorothioate 10-23DNAzyme specific to eIF4E gene mRNA 1059 was designed and synthesized, and its inhibition effects on the expression of eIF4E gene in Hep-2 cells were observed.@*RESULT@#The expression of eIF4E gene was remarkable depressed after Hep-2 cells was transfected by DNAzyme. The level of inhibiting eIF4E in hep-2 cells transfected by DNAzyme was lower than that by only lipofectamine 2000 transfected and Hep-2.@*CONCLUSION@#The expression of eIF4E gene in Hep-2 cells 10-23DNAzyme can be highly blocked. It is a specific and effective gene therapeutic means.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Single-Stranded
/
RNA, Messenger
/
Transfection
/
Genetic Therapy
/
Gene Expression
/
Laryngeal Neoplasms
/
Apoptosis
/
DNA, Catalytic
/
Eukaryotic Initiation Factor-4E
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2007
Type:
Article
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