The role of protein kinase C and activator protein-1 signal cascade in the regulation of expression of interleukin-5 in T lymphocyte of allergic rhinitis / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 71-74, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-748895
ABSTRACT
OBJECTIVE@#To explore the role of protein kinase C (PKC) and activator protein-1 (AP-1) in the signal transduction cascade in the expression of interleukin-5 (IL-5) by peripheral blood T lymphocytes from allergic rhinitis.@*METHOD@#Twenty-five allergic rhinitis patients and twenty-three deflection of nasal septum(DNS) persons in the study. T lymphocytes were isolated and purified from peripheral blood of each person and randomly divided into 3 groups group A, blank (DNS and AR); group B, PKC excitomotor 12-myristate 13-acetate (DNS PMA and AR PMA); group C , PMA and curcumin (DNS PMA/curcumin and AR PMA/curcumin). ELISA was used to assess the expression of IL-5 in supernatants, and immunocytochemical staining for the activation of AP-1.@*RESULT@#The percentage of cells of active AP-1, IL-5 protein in supernatants of allergic rhinitis T lymphocytes stimulated with PMA were significantly higher than those of allergic rhinitis T lymphocytes stimulated without PMA (P < 0.01) and with PMA and curcumin (P < 0.01); than those of deflection of nasal septum T lymphocytes stimulated with PMA (P < 0.01), with PMA and curcumin (P < 0.01) and without PMA. The percentage of cells of active AP-1, IL-5 protein in supernatants of allergic rhinitis T lymphocytes stimulated with PMA and curcumin were significantly lower than those of allergic rhinitis T lymphocytes stimulated with PMA (P < 0.01); but significantly higher than those of allergic rhinitis T lymphocytes stimulated without PMA and those of deflection of nasal septum T lymphocytes stimulated. There were good positive correlation between the percentage of cells of active AP-1 and IL-5 protein in supernatants (r = 0.92, P < 0.01).@*CONCLUSION@#AP-1 may participate in the signal transduction of PKC-triggered expression of IL-5 in allergic rhinitis T lymphocytes. This suggests the activation of PKC/AP-1 signal transduction cascade of T lymphocytes may play an important role in the pathogenesis of allergic rhinitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Congenital Abnormalities
/
Protein Kinase C
/
T-Lymphocytes
/
Signal Transduction
/
Rhinitis
/
Interleukin-5
/
Transcription Factor AP-1
/
Allergy and Immunology
/
Metabolism
/
Nasal Septum
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2007
Type:
Article
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