Gene expression profiles in human nasal polyps studied by DNA microarray / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To explore the changes in gene expressions in the human nasal polyp.@*METHOD@#A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.@*RESULTS@#The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.@*CONCLUSION@#Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.