Inhibiting effects of TFPI-2 recombinant adenovirus on invasion of laryngeal squamous carcinoma / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To study the effects of adenovirus-mediated tissue factor pathway inhibitor-2 gene on the invasion of laryngeal squamous cancer.@*METHOD@#Ad-TFPI-2 was transfected into laryngeal squamous cancer (Hep-2) cell. Western-blot was used to test the TFPI-2 protein expression and Boyden Chamber experiment was used to examine the invasive ability of Hep-2 cells. Furthermore, the Ad-TFPI-2 infected Hep-2 cells were subcutaneously inoculated in nude mice and the tumor formation capability were observed.@*RESULT@#Ad-TFPI-2 was identified correctly by endonuclease and sequencing and the virus titer was 2.8 x 10(13) PFU/L. In the Hep-2 cells of treated group, the TFPI-2 protein expression was increased while the invasive capability was descent. The tumor formation capability was also decreased in the treated group nude mouse model.@*CONCLUSION@#TFPI-2 recombinant adenovirus can effectively inhibit the invasive capability of laryngeal squamous cancer.