Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 921-924, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-749263
ABSTRACT
OBJECTIVE@#To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.@*METHOD@#The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.@*RESULT@#The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.@*CONCLUSION@#The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Calibration
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Pyroglyphidae
/
Allergy and Immunology
/
Indicators and Reagents
/
Methods
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2013
Type:
Article
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