Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 178-181, 2014.
Article
in Zh
| WPRIM
| ID: wpr-749401
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.@*METHOD@#The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.@*RESULT@#Suicide genes were expressed stably in CNE-2 cells.@*CONCLUSION@#We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.
Full text:
1
Index:
WPRIM
Main subject:
Carcinoma
/
Nasopharyngeal Neoplasms
/
Artificial Gene Fusion
/
Cell Line, Tumor
/
Genes, Transgenic, Suicide
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Nasopharyngeal Carcinoma
/
Genetics
/
Methods
Limits:
Humans
Language:
Zh
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2014
Type:
Article