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Accuracy of serum-specific IgE test with microfluidic array enzyme-linked immunosorbent assay for diagnosing inhalant allergen sensitization in asthma and/or rhinitis allergy patients in Jakarta, Indonesia
Asia Pacific Allergy ; (4): e10-2018.
Article in English | WPRIM | ID: wpr-750123
ABSTRACT

BACKGROUND:

Asthma and allergic rhinitis are a global health burden. Inhalant allergens worsen the symptoms and clinical manifestations of asthma and allergic rhinitis. Skin prick test is the gold standard for diagnosing allergen sensitization but is associated with some limitations. In contrast, in vitro serum-specific immunoglobulin E (SSIgE) test is convenient and is not associated with an anaphylactic risk.

OBJECTIVE:

The present study compared the accuracy of the SSIgE test by using microfluidic array enzyme-linked immunosorbent assay (ELISA) with that of the skin prick test for diagnosing inhalant allergen sensitization in patients with asthma and/or allergic rhinitis.

METHODS:

This diagnostic study included patients with asthma and/or allergic rhinitis. Of these, 100 patients underwent the SSIgE test for diagnosing sensitization to house dust mites (Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Blomia tropicalis), dog dander, cat dander, and cockroach allergen. All the patients also underwent the skin prick test for diagnosing allergen sensitization. The sensitivity, specificity, predictive value, and likelihood ratio (LR) of the SSIgE test were evaluated for each allergen.

RESULTS:

Sensitivity of the SSIgE test for diagnosing house dust mite sensitization was 48%–77%, with the highest sensitivity (77%) observed for diagnosing D. farinae sensitization. Specificity of the SSIgE test for diagnosing house dust mite sensitization was 64%–95%, with the highest specificity (95%) observed for diagnosing B. tropicalis sensitization. Although the SSIgE test showed high specificity and LR+ for diagnosing cockroach allergen sensitization, it showed low sensitivity (12%). Moreover, the SSIgE test showed high specificity (89%) but low sensitivity (3%) for diagnosing dog dander sensitization and high specificity (88%) but low sensitivity (10%) for diagnosing cat dander sensitization.

CONCLUSION:

The SSIgE test using microfluidic array ELISA shows moderate accuracy for diagnosing house dust mite sensitization and low accuracy for diagnosing cockroach allergen and dog and cat dander sensitization.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Asthma / Skin / In Vitro Techniques / Immunoglobulin E / Immunoglobulins / Enzyme-Linked Immunosorbent Assay / Skin Tests / Allergens / Rhinitis / Global Health Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Country/Region as subject: Asia Language: English Journal: Asia Pacific Allergy Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Asthma / Skin / In Vitro Techniques / Immunoglobulin E / Immunoglobulins / Enzyme-Linked Immunosorbent Assay / Skin Tests / Allergens / Rhinitis / Global Health Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Country/Region as subject: Asia Language: English Journal: Asia Pacific Allergy Year: 2018 Type: Article