Effect of Yinao Jieyu Compound Formula on Intestinal Microflora in Stress Rats of Post-stroke / 世界科学技术-中医药现代化

ABSTRACT

Objective: To explore the mechanism of Yinao Jieyu Compound Formula in the treatment of post-stroke stressrats from the perspective of intestinal flora changes. Methods: A total of 48 male clean SD rats were randomly divided into4 groups sham-operation group, post-stroke stress group, western medicine group and traditional Chinese medicinegroup, with 12 rats in each group. In the sham-operation group, only the free blood vessels were not inserted into thethread embolus. The rats in post-stroke stress group, the western medicine group and the Chinese medicine group weretreated as follows after 1 week of middle cerebral artery occlusion, the rat model of PSD was prepared by using chronicrestraint stress and single cage feeding. The 7 th day from the beginning of the construction of the cerebral stroke model, the gavage treatment began to be done. The western medicine group was given Fluoxetine Hydrochloride Capsules2.33 mg/ (kg.d), and the Chinese medicine group was given 9.92 g/ (kg·D) . The rest of the rats were gavage with 10 mL/kgdistilled water, 1 time/day. The behavioral changes on the 14 th day after post-stroke were observed and the microbialcommunity structure and composition was analyzed by Illumina-MiSeq high throughput sequencing technique. Results:Compared with the sham operation group, the sucrose water preference, open field score and swimming struggle time ofthe post-stroke stress group were significantly decreased (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01), and the proportions ofAllobaculum, Sutterella, Dorea and rc4-4 in the feces decreased significantly (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01, P ＜ 0.05) .The proportion of Oscillospira increased significantly (P ＜ 0.01, P ＜ 0.05, P ＜ 0.01) . Compared with the post-strokestress group, the horizontal scores and swimming struggling time of the rats in the Chinese medicine group weresignificantly increased (P ＜ 0.05, P ＜ 0.05), and the proportions of the 6 species of Lactobacillus and Bacteroides weresignificantly increased in the feces. The difference between Lactobacillus and Bacteroides was the most obvious with27.91％ vs 5.96％ and 24.21％ vs 9.62％ (P ＜ 0.01, P ＜ 0.01) . The proportion of Oscillospira was significantly decreased (P ＜ 0.05) . Compared with the western medicine group, there was no significant difference in the behavioral scores of therats in the traditional Chinese medicine group (P＞ 0.05), and the proportion of Bacteroides and rc4-4 in the feces wassignificantly increased (P ＜ 0.01, P ＜ 0.05) . Conclusion: Yinao Jieyu Compound Formula can alleviate similar depressivesymptoms in post-stroke stress rats by increasing the content of Lactobacillus and Bacteroides in the intestine of rats.