Optimization of Extraction Technology of Shen Qi Jie Yu Prescription with Orthogonal Test / 世界科学技术-中医药现代化

ABSTRACT

Objective: To optimize the extraction technology of Shen Qi Jie Yu prescription. Methods: High performance liquid chromatography (HPLC) method was selected to determine the content of hesperidin of Shen Qi Jie Yu prescription of traditional Tang and granules. Decoction time, times and add water were used as the examining factors. L9 (34) orthogonal test was used as the best condition of extraction. Results: The hesperidin content in traditional Tang of Shen Qi Jie Yu prescription was significantly higher than granules. The order of the effect of hesperidin content is decoction time＞ decoction times＞add water. There is a significant difference in the number of boiling times. The best decoction condition of Shen Qi Jie Yu Prescription was 3 times for decoction, with 0.5 hours of decoction and 7 times of water addition. Conclusion: The high performance liquid chromatography of Shen Qi Jie Yu Prescription is the quantitative method and the preferred decoction technique of Shen Qi Jie Yu prescription is stable and reliable.