Significance of endoplasmic reticulum stress_associated gene tribbles pseudokinase 3 in the long_term brain injury in developing epileptic rats / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the significance of endoplasmic reticulum stress _associated gene tri_bbles pseudokinase 3(TRIB3)in the long_term brain injury in rats with developing epilepy. Methods Thirty male SD rats aged 21 days were randomly divided into the control group and the epilepsy group,15 rats in each group. The rats in the epilepsy group were intraperitoneally injected with kainic acid(10 mg/kg)to induce seizures,while the rats in the control group were injected with the equal volume of 9 g/L saline. The rats in two groups were euthanized at 30 d after kainic acid administration. The damage to the ultrastructure of the cortex were observed by using transmission elec_tron microscopy. Neuronal apoptosis in the cortex of rats was detected by terminal_deoxynucleoitidyl transferase media_ted nick end labeling( TUNEL)assay. The expression and localization of glucose regulated protein 78( xRP78), CCAAT/enhancer binding protein _ homologous protein( CHOP ),TRIB3,and the activation of protein kinase B (AKT)in the cortex were examined by using Western blot analysis and immunohistochemistry. Results Compared with the control group,the different ultrastructural changes were observed in the cortex in the epilepsy group rats. TUNEL assay indicated that the number of apoptosis cells of cortex in the epilepsy group was increased. The protein levels of xRP78 and TRIB3 were upregulated in the cortex of the epileptic rats(1. 280 ± 0. 272,1. 725 ± 0. 570),com_pared with the control group(1. 000 ± 0. 000,1. 000 ± 0. 000),and the differences were statistically significant( all P<0. 05). There was no significant change in CHOP protein between the control group and the epilepsy group. The level of phosphorylated AKT(p_AKT)was decreased in the cortex of the epilepsy group(0. 150 ± 0. 047),compared with the control group(1. 000 ± 0. 000),and the difference was statistically significant(P<0. 05). Conclusions The brain injury caused by epilepsy in the developing rats can sustain to the stage of mature rats,and the endoplasmic reti_culum stress_associated gene TRIB3 is involved in the pathogenesis of long_term brain damage in the rats with deve_loping epilepsy.