Peripheral serum levels of MMP—2 ,MMP—9 and TIMP—1 in CHF patients and their relationship with prognosis / 心血管康复医学杂志

ABSTRACT

Objective:To detect peripheral serum levels of matrix metalloproteinase (MMP)—2 ,MMP—9 and tissue in—hibitor of metalloproteinases (TIMP)—1 in CHF patients ,and analyse their relationship with CHF prognosis .Meth—ods A total of 200 CHF patients ,who were hospitalized in our hospital from Mar 2015 to Mar 2017 ,were enrolled as CHF group ;according to NYHA classification ,they was further divided into class II group (n＝ 78 ) ,class III group (n＝68) and class IV group (n＝ 54 ) ;according to prognostic condition ,CHF group was divided into good prognosis group (n＝126) and poor prognosis group (n＝74).Another 150 healthy subjects were enrolled as healthy control group .Peripheral serum levels of MMP—2 ,MMP—9 and TIMP—1 were measured and compared among all group .Results: Compared with healthy control group , there were significant rise in peripheral serum levels of MMP—2 and MMP—9 ,and significant reduction in TIMP—1 level in CHF group , P＝ 0.001 all.Along with NYHA class rose , peripheral serum levels of MMP—2 and MMP—9 gradually increased , and TIMP—1 level gradually de—creased ,there existed significant difference between any two subgroups , P＝0.001 all.Compared with good prog—nosis group ,there were significant rise in peripheral serum levels of MMP—2 [ (47.72 ± 9.57) ng／ml vs.(57. 42 ± 8.63) ng／ml] ,MMP—9 [ (50. 63 ± 8. 95) ng／ml vs.(62.83 ± 9. 67) ng／ml] ,and significant reduction in TIMP—1 level [ (29. 68 ± 6. 43) ng／ml vs.(20.74 ± 4.07) ng／ml] , P＝0.001 all.AUCs of MMP—2 ,MMP—9 and TIMP—1 judging poor prognosis was 0.689 ,0.683 and 0. 871 respectively ;sensitivity and specificity of MMP—2≥51. 13ng／ml , MMP—9≥57. 67ng／ml ,TIMP—1＜24. 79ng／ml predicting poor prognosis in CHF patients were 59.12% and 80. 93%, 67. 57% and 73.86%,94.19% and 81.79% respectively ,and their Youden＇s indexes were 0. 616 ,0.623 and 0. 516 respectively .Kaplan—Meier analysis indicated that according to two—year poor prognosis rate ,the high level MMP—2 patients was significantly higher than that of low level patients (78.05% vs.8.47%) ,and that of high level MMP—9 patients was significantly higher than that of low level patients (72.09% vs.10.53%) ,and that of low level TIMP—1 patients was significantly higher than that of high level patients (87.18% vs.4.92%) , P＝0.001 all.Con—clusion There are significant rise in MMP—2 and MMP—9 levels ,and significant reduction in TIMP—1 level in periph—eral blood of CHF patients .They significantly affect patients＇prognosis ,which may be effective markers predicting prognosis of CHF .