The expression and significance of miR-29b and IGF-1 in type 2 diabetes mellitus patients with de-pression / 中国医师杂志

ABSTRACT

Objective To detect the levels of serum micro RNA (miRNA) -29b and insulin growth factor-1 (IGF-1) in patients with type 2 diabetes mellitus (T2DM) and depression, and to analyze their clinical significance. Methods 504 patients with T2DM admitted to our hospital from February 2016 to February 2018 were selected, among them, 264 patients without depression, the other 240 patients with de-pression were selected as the study group, and 200 patients with physical examination were selected as the control group. Serum triglyceride (TG), cholesterol (TC), low density lipoprotein cholesterol (LDL), high density lipoprotein cholesterol ( HDL), glycosylated hemoglobin ( HbA1c), fasting blood glucose ( FPG) were measured by automatic biochemical analyzer. Levels of serum IGF-1 were measured by auto-matic chemiluminescence instrument in all subjects. The expression of miR-29b in serum was detected by quantitative real-time polymerase chain reaction ( Qrt-PCR ) . All subjects were scored with 24 Hamilton Scales (HAMD). Results The serum TC, TG, LDL, FPG, HbA1c, and HAMD scores of patients with depression in T2DM were significantly higher than those of control group and patients with T2DM ( P <0. 05);the serum TC, TG, FPG, HbA1c of patients with T2DM were significantly higher than those of the control group (P<0. 05). The levels of serum miR-29b in patients with T2DM and depression were signifi-cantly lower than those in patients with T2DM and control group (P<0. 05), while the level of IGF-1 was significantly higher than that in patients with T2DM and in control group (P<0. 05); the level of serum miR-29b in patients with T2DM was significantly lower than that in control group (P<0. 05), while the level of IGF-1 was significantly higher than that in control group (P<0. 05). The levels of serum miR-29b in patients with severe depression and T2DM were significantly lower than those in patients with mild or moderate depression (P<0. 05), while the level of IGF-1 showed the opposite (P<0. 05). There was a significant negative correlation between serum miR-29b and IGF-1 level in patients with T2DM and depres-sion (P<0. 05). Logistic regression analysis showed that high levels of serum HbA1c (≥11. 06％) and IGF-1 (≥25. 74 ng/ml) were risk factors for depression in patients with T2DM, and high level of serum miR-29b (≥0. 39) was protective (P<0. 05). Conclusions The serum levels of IGF-1 increased and miR-29b decreased in patients with type 2 diabetes mellitus and depression. Serum levels of miR-29b were negatively correlated with the levels of IGF-1. High levels of IGF-1 were risk factors for depression in pa-tients with type 2 diabetes mellitus, and high levels of miR-29b were protective factors.