Expression and significance of nuclear factor of activated T-cells cytoplasmic 1 in hepatocellular carcinoma / 中华肝胆外科杂志

ABSTRACT

Objective To investigate the function of activated T cell nucleus factor 1 (NFATc1) in liver cancer tissues and the correlations between (NFATc1 and prognosis,and the effect of NFATc1 on liver cancer cell migration.Methods The hepatobiliary surgery department of Tianjin First Central Hospital collected 33 patients with hepatocellular carcinoma from January to June 2015,and studied the expression of NFATc1 in liver cancer and adjacent tissues and the prognosis of patients.HCC cell HepG2 were randomly divided into blank control group (without any intervention),interference group (infected with lentivirus interference sequence,down-regulated expression of NFATc1),and negative control group (infected with lentivirus negative control sequence).The relative expression of NFATc1 in the patients and the three groups were detected by real-time fluorescence quantitative polymerase chain reaction (rt-pcr),and the expressions of NFATc1,n-cadherin and e-cadherin in the three groups were detected by Western blot,and the migration of the cells was detected by Transwell.Results The mRNA level of NFATc1 in liver cancer tissues was higher than that in adjacent tissues,and the difference was statistically significant (P ＜ 0.05).According to the median expression of NFATc1,the 33 patients were divided into high-expression group (n =17) and low-expression group (n =16).The survival rate of the high-expression group was better than that of the low-expression group,and the difference was statistically significant (P ＜ 0.05).The mRNA level of NFATc1 in the interference group was lower than that in the negative control group and the blank control group,and the difference was statistically significant (P ＜0.05).The mRNA levels of NFATc1 and n-cadherin in the interference group were lower than those in the negative control group and the blank control group,while the mRNA levels of e-cadherin were higher than those in the negative control group and the blank control group,with statistically significant differences (P ＜ 0.05).The number of migrative cells in the interference group was (24.0 ± 5.6),which was lower than that in the blank control group [(69.0 ±4.0)] and the negative control group [(73.0 ±4.4)],and the difference was statistically significant (P ＜0.05).Conclusions The expression of NFATc1 is increased in liver cancer,and high level of NFATc1 indicated poor prognosis in liver cancer patients.Low expression of NFATc1 reduces the migration ability of liver cancer cells.