Detection of Methicillin Resistance in Staphylococcus aureus by 2% NaCl Mueller-Hinton Agar and Mannitol Salt Agar / 감염
Korean Journal of Infectious Diseases
;
: 539-544, 1998.
Article
in Korean
| WPRIM
| ID: wpr-75557
ABSTRACT
BACKGROUND:
Methicillin-resistant Staphylococcus aureus (MRSA) has a heteroresistant nature, so methicillin resistance is influenced by various culture conditions, such as temperature, incubation time, and NaCl content in the medium. Mueller Hinton (MH) agar containing 2% NaCl and mannitol salt agar (MSA) with oxacillin disk were evaluated for the detection of methicillin resistance.METHODS:
Disk diffusion test on plain Mueller- Hinton (MH) agar, 2% NaCl MH agar, and MSA with 1 microgram oxacillin disk was performed in 70 Stap hylococcus aureus isolates. Oxacillin MIC was determined by E-test. As a gold standard of methicillin resistance, mecA gene was amplified by PCR and detected by agarose gel electrophoresis.RESULTS:
Plain MH agar could not detect heterogeneous resistance in 12 S. aureus isolates (18%), but 2% NaCl MH agar and MSA could correctly detect homogeneous and heterogeneous resistance. S. aureus isolates from stool have as much as 48% heterogeneous resistance, while those from non-stool specimen have 5%.CONCLUSION:
2% NaCl and MSA can be used reliably for accurate susceptibility testing of methicillin resistance in routine laboratory.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxacillin
/
Staphylococcus
/
Staphylococcus aureus
/
Polymerase Chain Reaction
/
Methicillin Resistance
/
Agar
/
Diffusion
/
Electrophoresis, Agar Gel
/
Methicillin-Resistant Staphylococcus aureus
/
Mannitol
Type of study:
Diagnostic study
Language:
Korean
Journal:
Korean Journal of Infectious Diseases
Year:
1998
Type:
Article
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