Role of H1 receptor in inflammatory response during ventilator-induced lung injury in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the role of H1 receptor in inflammatory responses during ventila-tor-induced lung injury ( VILI) in rats. Methods Thirty clean-grade healthy male Sprague-Dawley rats, aged 9-10 weeks, weighing 250-300 g, were divided into 3 groups ( n=10 each) using a random number table method: control group (group C), VILI group (group V) and H1 receptor antagonist clemastine group ( group Cle) . The animals were anesthetized and tracheostomized, and the rats in group C kept spon-taneous breathing. The rats were mechanically ventilated for 4 h with the tidal volume of 40 ml/kg, respira-tory rate 40 breaths/min, inspiratory/expiratory ratio 1 1, and inspired oxygen fraction ratio 21％ in group V. In group Cle, clemastine 0. 9 mg/kg was intramuscularly injected at 30 min before anesthesia, the ani-mals were tracheostomized after being anesthetized, and then the rats were mechanically ventilated with the same ventilator settings as group V. The animals were sacrificed at 4 h of ventilation, bronchoalveolar lav-age fluid ( BALF) was collected for determination of concentrations of total protein, interleukin-6 ( IL-6) , tumor necrosis factor-alpha ( TNF-α) , and the lung specimens were obtained for microscopic examination of pathologic changes and for determination of wet/dry weight ratio ( W/D ratio ) . Results Compared with group C, the concentrations of total protein, IL-6 and TNF-αin BALF and W/D ratio were significantly in-creased in group V (P＜0. 05). Compared with group V, the concentrations of total protein, IL-6 and TNF-αin BALF and W/D ratio were significantly decreased in group Cle ( P＜0. 05) . The pathologic chan-ges of lung tissues were significantly attenuated in group Cle as compared with group V. Conclusion H1 receptor is involved in the process of inflammatory responses during VILI in rats.