The protective effect of interleukin-5 in septic mice / 中华内科杂志

ABSTRACT

To investigate the protective effect of interleukin-5 (IL-5) in septic mice,male C57BL/6 mice were divided into three groups including sepsis group with intraperitoneal injection of lipopolysaccharide (LPS,20 mg/kg),study group with combined IL-5 (1 μg per mouse) and LPS (20 mg/kg),control group with normal saline.Pathological changes were tested including lung,heart and kidney tissues.The serum cardiac troponin I (cTnI),serum creatinine (SCr),blood urea nitrogen (BUN),tumor necrosis factor α (TNFα),IL-6,IL-1β,and the chemokine (C-X-C motif) ligand 1(CXCL1) were measured and the survival at 7 d after treatment were recorded.Compared with sepsis group,tissue damages of lung,heart and kidney in IL-5 intervention group were ameliorated.The cTnI,SCr,BUN levels in study group were significantly decreased at 24 h (P＜0.05).In addition,TNFα was significantly decreased at 3 h (P＜0.05),and CXCL1 decreased at 12 h (P＜0.05),while IL-6 and IL-1β were similar at each time point (P＞0.05).The mortality at 7 d was less in IL-5 treatment group.In summary,IL-5 has a protective effect and reduces the inflammatory response in septic mice.