The influence of probiotics and synbiotics on intestinal inflammation and microbiota in mice with acute colitis / 中华内科杂志

ABSTRACT

Objective To investigate the effects of probiotics and synbiotics on inflammation and microbiota of acute colitis in mice.Methods C57BL/6J mice were divided into 4 groups randomly.Each group had 10 mice and was given 2.5％ dextran sulfate sodium (DSS) drinking water for 5 days other than the blank control group.Except for model control group,other two groups were administrated with probiotics and synbiotics,respectively.Probiotics was composed of Lactobacillus acidophilus,Lactobacillus rhamnosus and Bifidobacterium lactis,while synbiotics was composed of the aforementioned probiotics,inulin and galactooligosaccharide.Feces of different periods and mucosa samples were collected to analyze the differences of enteric flora by 16s rDNA sequencing.Results (1) Pathological scores in probiotics group and synbiotics group were 5.40±2.79 and 7.25±2.87,respectively,which were significantly lower than those in the model control group with scores 27.00 ± 7.94.Model control group,probiotics group and synbiotics group showed lower flora diversity,increased Bacteroides and decreased Faecalibacterium than blank control group.The mucosal microbiota was different from fecal flora in abundance and species for each group,and Mucispirillum was more common in mucosa.Conclusions Probiotics and synbiotics alleviate the inflammation of acute colitis in mice.Imbalance of beneficial genera to harmful genera is the characteristic of acute colitis.Supplementation of probiotics and synbiotics contributes to regulating the balance of intestinal microbiota.