Effect of different routes of Dexamethasone injection on aqueous humor concentration and blood glucose in type 2 diabetic rats / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

@#AIM: To evaluate the effect of different routes of dexamethasone administration on aqueous humor concentration and blood glucose in type 2 diabetic(T2DM)rats.<p>METHODS: The T2DM rats successfully modeled were randomly divided into the experimental group(dexamethasone sodium phosphate injection)and the control group(normal saline injection). Each group was randomly divided into three subgroups peribulbar injection group, post-bulbar injection group and subconjunctival injection group according to different drug delivery routes. Rat tail venous blood was collected 0-24h after injection and blood glucose concentration was determined by dipstick method. After injection, dexamethasone concentration in aqueous aqueous solution of both eyes was determined by HPLC at 0.5-24h.<p>RESULTS: Dexamethasone sodium phosphate or normal saline injection had no significant effect on blood glucose concentration in T2DM rats(<i>P</i>>0.05). After dexamethasone sodium phosphate was injected in different ways for 0.5-24h, rats in the experimental group received dexamethasone concentration peak under bulbar conjunctiva injection group(957.34±3.60ng/mL)>post-bulbar injection group(859.60±3.56ng/mL)>perbulbar injection group(732.38±4.56ng/mL).<p>CONCLUSION: Injection of dexamethasone sodium phosphate into bulbous injection, post-bulbar injection and subconjunctival injection had no significant effect on blood glucose of T2DM rats, while subconjunctival injection was simple and could reach higher drug concentration in a short time than that injected into bulbous injection and sub-bulbous injection.