Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain
Protein & Cell
;
(12): 470-476, 2011.
Article
in English
| WPRIM
| ID: wpr-757075
ABSTRACT
Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphoproteins
/
Plasmids
/
Protein Binding
/
Endosomes
/
Virion
/
Virology
/
Virus Replication
/
Transfection
/
Down-Regulation
Limits:
Humans
Language:
English
Journal:
Protein & Cell
Year:
2011
Type:
Article
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