Targeted deletion of mouse Rad1 leads to deficient cellular DNA damage responses
Protein & Cell
;
(12): 410-422, 2011.
Article
in English
| WPRIM
| ID: wpr-757086
ABSTRACT
The Rad1 gene is evolutionarily conserved from yeast to human. The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G(2)/M checkpoint activation. In this study, mouse embryonic stem (ES) cells with a targeted deletion of Mrad1, the mouse ortholog of this gene, were created to evaluate its function in mammalian cells. Mrad1 (-/-) ES cells were highly sensitive to ultraviolet-light (UV light), hydroxyurea (HU) and gamma rays, and were defective in G(2)/M as well as S/M checkpoints. These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light, HU and gamma rays, and for mediating G(2)/M and S/M checkpoint controls. We further demonstrated that Mrad1 plays an important role in homologous recombination repair (HRR) in ES cells, but a minor HRR role in differentiated mouse cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Ultraviolet Rays
/
DNA Damage
/
Cell Division
/
G2 Phase
/
Gene Deletion
/
Cell Proliferation
/
DNA Repair
/
Exonucleases
Limits:
Animals
Language:
English
Journal:
Protein & Cell
Year:
2011
Type:
Article
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