Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner
Protein & Cell
; (12): 150-160, 2011.
Article
in En
| WPRIM
| ID: wpr-757118
Responsible library:
WPRO
ABSTRACT
Angiogenesis, a process by which the preexisting blood vasculature gives rise to new capillary vessels, is associated with a variety of physiologic and pathologic conditions. However, the molecular mechanism underlying this important process remains poorly understood. Here we show that histone deacetylase 6 (HDAC6), a microtubule-associated enzyme critical for cell motility, contributes to angiogenesis by regulating the polarization and migration of vascular endothelial cells. Inhibition of HDAC6 activity impairs the formation of new blood vessels in chick embryos and in angioreactors implanted in mice. The requirement for HDAC6 in angiogenesis is corroborated in vitro by analysis of endothelial tube formation and capillary sprouting. Our data further show that HDAC6 stimulates membrane ruffling at the leading edge to promote cell polarization. In addition, microtubule end binding protein 1 (EB1) is important for HDAC6 to exert its activity towards the migration of endothelial cells and generation of capillary-like structures. These results thus identify HDAC6 as a novel player in the angiogenic process and offer novel insights into the molecular mechanism governing endothelial cell migration and angiogenesis.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
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Cell Movement
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Cells, Cultured
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Chickens
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Cell Polarity
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Neovascularization, Physiologic
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Cell Biology
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Endothelial Cells
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Histone Deacetylase 6
Limits:
Animals
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Humans
Language:
En
Journal:
Protein & Cell
Year:
2011
Type:
Article