Apaf1 inhibition promotes cell recovery from apoptosis
Protein & Cell
;
(12): 833-843, 2015.
Article
in English
| WPRIM
| ID: wpr-757189
ABSTRACT
The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
HeLa Cells
/
Doxorubicin
/
Cell Hypoxia
/
Adenosine Triphosphate
/
Apoptosis
/
Cell Line, Tumor
/
Microscopy, Electron, Transmission
/
Apoptotic Protease-Activating Factor 1
/
Genetics
Limits:
Humans
Language:
English
Journal:
Protein & Cell
Year:
2015
Type:
Article
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