Bisindoylmaleimide I enhances osteogenic differentiation
Protein & Cell
;
(12): 311-320, 2012.
Article
in English
| WPRIM
| ID: wpr-757265
ABSTRACT
The Wnt/β-catenin and bone morphogenetic proteins (BMPs) pathways play important roles in controlling osteogenesis. Using a cell-based kinase inhibitor screening assay, we identified the compound bisindoylmaleimide I (BIM) as a potent agonist of the cytosolic β-catenin accumulation in preosteoblast cells. Through suppressing glycogen synthase kinase 3β enzyme activities, BIM upregulated β-catenin responsive transcription and extended duration of BMP initiated signal. Functional analysis revealed that BIM promoted osteoblast differentiation and bone formation. The treatment of human mesenchymal stem cells with BIM promoted osteoblastogenesis. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by integration of the cellular signaling pathways.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
Signal Transduction
/
Cell Differentiation
/
Chemistry
/
Bone Morphogenetic Proteins
/
Cell Biology
/
Glycogen Synthase Kinase 3
/
RNA, Small Interfering
/
RNA Interference
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Protein & Cell
Year:
2012
Type:
Article
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