Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro
Protein & Cell
;
(12): 153-159, 2012.
Article
in English
| WPRIM
| ID: wpr-757300
ABSTRACT
The sigma-1 receptor is a molecular chaperone protein highly enriched in the brain. Recent studies linked it to many diseases, such as drug addition, Alzheimer's disease, stroke, depression, and even cancer. Sigma-1 receptor is enriched in lipid rafts, which are membrane microdomains essential in signaling processes. One of those signaling processes is ADAM17- and ADAM10-dependent ectodomain shedding. By using an alkaline phosphatase tagged substrate reporter system, we have shown that ADAM10-dependent BTC shedding was very sensitive to both membrane lipid component change and sigma-1 receptor agonist DHEAS treatment while ADAM17-dependent HB-EGF shedding was not; and overexpression of sigma-1 receptor diminished ADAM17- and ADAM10-dependent shedding. Our results indicate that sigma-1 receptor plays an important role in modifying the function of transmembrane proteases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gene Expression
/
Chlorocebus aethiops
/
Receptors, sigma
/
COS Cells
/
Membrane Microdomains
/
Intercellular Signaling Peptides and Proteins
/
ADAM Proteins
/
Amyloid Precursor Protein Secretases
/
HEK293 Cells
/
Betacellulin
Limits:
Animals
/
Humans
Language:
English
Journal:
Protein & Cell
Year:
2012
Type:
Article
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