Engineering a zinc binding site into the de novo designed protein DS119 with a βαβ structure
Protein & Cell
;
(12): 1006-1013, 2011.
Article
in English
| WPRIM
| ID: wpr-757311
ABSTRACT
Functional proteins designed de novo have potential application in chemical engineering, agriculture and healthcare. Metal binding sites are commonly used to incorporate functions. Based on a de novo designed protein DS119 with a βαβ structure, we have computationally engineered zinc binding sites into it using a home-made searching program. Seven out of the eight designed sequences tested were shown to bind Zn(2+) with micromolar affinity, and one of them bound Zn(2+) with 11 stoichiometry. This is the first time that metalloproteins with an α, β mixed structure have been designed from scratch.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Zinc
/
Binding Sites
/
Computer Simulation
/
Recombinant Proteins
/
Molecular Sequence Data
/
Kinetics
/
Magnetic Resonance Spectroscopy
/
Protein Engineering
/
Models, Molecular
Language:
English
Journal:
Protein & Cell
Year:
2011
Type:
Article
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