miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma
Protein & Cell
;
(12): 899-912, 2016.
Article
in English
| WPRIM
| ID: wpr-757360
ABSTRACT
The BCL6 (B-Cell Lymphoma 6) gene is a proto-oncogene that is often expressed in diffuse large B-cell lymphomas (DLBCLs). BCL6 loss of function can kill DLBCL cells, demonstrating that BCL6 is necessary for the survival of DLBCL cells and could be a therapeutic target. In this study, we found that BCL6 protein levels were consistently upregulated in DLBCL tissues, whereas its mRNA levels varied randomly in tissues, suggesting that a post-transcriptional mechanism was involved in BCL6 regulation. We used bioinformatics analysis to search for miRNAs, which potentially target BCL6, and identified specific targeting sites for miR-10a in the 3'-untranslated region (3'-UTR) of BCL6. We further identified an inverse correlation between miR-10a levels and BCL6 protein levels, but not mRNA levels, in DLBCL tumor tissue samples. By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3'-UTR of the BCL6 transcript and regulated BCL6 expression. Furthermore, we demonstrated that negatively regulating BCL6 by miR-10a suppressed the proliferation and promoted apoptosis of DLBCL cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Gene Expression Regulation, Neoplastic
/
Lymphoma, Large B-Cell, Diffuse
/
Apoptosis
/
3' Untranslated Regions
/
MicroRNAs
/
Cell Line, Tumor
/
Cell Proliferation
/
Proto-Oncogene Proteins c-bcl-6
/
Gene Knockdown Techniques
Limits:
Humans
Language:
English
Journal:
Protein & Cell
Year:
2016
Type:
Article
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