Analysis of the p53/CEP-1 regulated non-coding transcriptome in C. elegans by an NSR-seq strategy
Protein & Cell
;
(12): 770-782, 2014.
Article
in English
| WPRIM
| ID: wpr-757473
ABSTRACT
In recent years, large numbers of non-coding RNAs (ncRNAs) have been identified in C. elegans but their functions are still not well studied. In C. elegans, CEP-1 is the sole homolog of the p53 family of genes. In order to obtain transcription profiles of ncRNAs regulated by CEP-1 under normal and UV stressed conditions, we applied the 'not-so-random' hexamers priming strategy to RNA sequencing in C. elegans, This NSR-seq strategy efficiently depleted rRNA transcripts from the samples and showed high technical replicability. We identified more than 1,000 ncRNAs whose apparent expression was repressed by CEP-1, while around 200 were activated. Around 40% of the CEP-1 activated ncRNAs promoters contain a putative CEP-1-binding site. CEP-1 regulated ncRNAs were frequently clustered and concentrated on the X chromosome. These results indicate that numerous ncRNAs are involved in CEP-1 transcriptional network and that these are especially enriched on the X chromosome in C. elegans.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Ultraviolet Rays
/
X Chromosome
/
Binding Sites
/
Tumor Suppressor Protein p53
/
Promoter Regions, Genetic
/
Sequence Analysis, RNA
/
Caenorhabditis elegans
/
RNA, Untranslated
/
Caenorhabditis elegans Proteins
Limits:
Animals
Language:
English
Journal:
Protein & Cell
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS