The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines
Protein & Cell
;
(12): 846-854, 2012.
Article
in English
| WPRIM
| ID: wpr-757852
ABSTRACT
The evolutionarily conserved RNA Polymerase II Rpb4/7 sub-complex has been thoroughly studied in yeast and impacts gene expression at multiple levels including transcription, mRNA processing and decay. In addition Rpb4/7 exerts differential effects on gene expression in yeast and Rpb4 is not obligatory for yeast (S. cerevisiae) survival. Specialised roles for human (hs) Rpb4/7 have not been extensively described and we have probed this question by depleting hsRpb4/7 in established human cell lines using RNA interference. We find that Rpb4/7 protein levels are inter-dependent and accordingly, the functional effects of depleting either protein are co-incident. hsRpb4/7 exhibits gene-specific effects and cells initially remain viable upon hsRpb4/7 depletion. However prolonged hsRpb4/7 depletion is cytotoxic in the range of cell lines tested. Protracted cell death occurs by an unknown mechanism and in some cases is accompanied by a pronounced elongated cell morphology. In conclusion we provide evidence for a gene-specific role of hsRpb4/7 in human cell viability.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA Polymerase II
/
HeLa Cells
/
Cell Line
/
Cell Nucleus
/
Cell Survival
/
Gene Expression Profiling
/
RNA, Small Interfering
/
RNA Interference
/
Genetics
Limits:
Humans
Language:
English
Journal:
Protein & Cell
Year:
2012
Type:
Article
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