Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Journal of Veterinary Science
;
: 35-43, 2018.
Article
in English
| WPRIM
| ID: wpr-758781
ABSTRACT
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All nsp4 genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that nsp4 genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 nsp4 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Variation
/
Virulence
/
Protein Processing, Post-Translational
/
Classification
/
Clone Cells
/
Viral Nonstructural Proteins
/
Sequence Analysis
/
Protein Folding
/
Rotavirus
/
Enterotoxins
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Journal of Veterinary Science
Year:
2018
Type:
Article
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