Your browser doesn't support javascript.
loading
Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge
Journal of Veterinary Science ; : 232-241, 2018.
Article in English | WPRIM | ID: wpr-758797
ABSTRACT
Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccine can protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologous recombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effect of this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutination inhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation were increased significantly more (p 0.05). Moreover, the novel strain provides 60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study, a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd (pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Poultry / Salmonella / Suicide / Bacteria / Newcastle disease virus / Immunoglobulin A, Secretory / Immunoglobulin G / Vaccines / Lymphocytes Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2018 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Poultry / Salmonella / Suicide / Bacteria / Newcastle disease virus / Immunoglobulin A, Secretory / Immunoglobulin G / Vaccines / Lymphocytes Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2018 Type: Article