Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Journal of Veterinary Science
;
: 216-231, 2018.
Article
in English
| WPRIM
| ID: wpr-758798
ABSTRACT
Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonoses. Pathogenesis of the B. abortus infection is complicated, and several researchers have attempted to elucidate the infection mechanism of B. abortus. While several proteins have been revealed as pathogenic factors by previous researchers, the underlying mechanism of B. abortus infection is unresolved. In this study, we identified proteins showing different expression levels in B. abortus mutants with different biological characteristics that were generated by random insertion of a transposon. Five mutants were selected based on biological characteristics, in particular, their growth features. Total proteins of mutant and wild-type B. abortus were purified and subjected to two-dimensional gel electrophoresis. Thirty protein spots of each mutant with expression increases or decreases were selected; those with a change of more than 2-fold were compared with the wild-type. Selected spots underwent liquid chromatography tandem mass spectrometry for peptide analysis. DnaK and ClpB, involved in protein aggregation, increased. SecA and GAPDH, associated with energy metabolism, decreased in some mutants with a growth rate slower than that of the wild-type. Mutants with slower growth showed a decrease in energy metabolism-related proteins, while mutants with faster growth showed an increase in pathogenicity-related proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Population Characteristics
/
Brucella
/
Brucella abortus
/
Brucellosis
/
Electrophoresis, Gel, Two-Dimensional
/
Zoonoses
/
Chromatography, Liquid
/
Sequence Analysis, Protein
/
Energy Metabolism
/
Tandem Mass Spectrometry
Type of study:
Prognostic study
Language:
English
Journal:
Journal of Veterinary Science
Year:
2018
Type:
Article
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