Clostridium botulinum spores in Polish honey samples
Journal of Veterinary Science
;
: 635-642, 2018.
Article
in English
| WPRIM
| ID: wpr-758850
ABSTRACT
The aim of this study was an examination of 240 multifloral honey samples collected from Polish apiaries to determine Clostridium botulinum occurrence. Honey was collected from apiaries directly after the extraction process. Samples were inoculated by using the dilution and centrifugation method. Suspected isolates were examined by using mouse bioassay, polymerase chain reaction (PCR), and real-time PCR methods. C. botulinum type A and B strains were detected in 5 of 240 examined honey samples (2.1%). Bacterial strains were also detected that were phenotypically similar to C. botulinum but that did not exhibit the ability to produce botulinum toxins and did not show the presence of the botulinum cluster (ntnh and bont genes) or expression of the ntnh gene. The methods used in the examination, especially the expression analysis of ntnh gene, enabled specific analysis of suspected strains and could be used routinely in environmental isolate analyses of C. botulinum occurrence.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spores
/
Biological Assay
/
Botulinum Toxins
/
Centrifugation
/
Polymerase Chain Reaction
/
Clostridium
/
Clostridium botulinum
/
Real-Time Polymerase Chain Reaction
/
Honey
/
Methods
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2018
Type:
Article
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